A Comparative Analysis of NOX4 Protein Expression in Malignant and Non-Malignant Thyroid Tumors

The comparative analysis of the expression reactive oxygen species-generating NADPH oxidase NOX4 from TCGA data shows that transcript is upregulated in papillary thyroid carcinomas (PTC)-BRAFV600E tumors compared to PTC-BRAFwt tumors. However, a at protein level malignant and non-malignant missing. We explored by immunohistochemistry staining (28 classical forms PTC (C-PTC), 17 follicular variants (F-PTC), three anaplastic (ATCs)) (six lymphocytic thyroiditis, four Graves’ disease, ten goiters, 20 hyperplasias). detected BRAFV600E mutation Sanger sequencing digital droplet PCR. results show was found be higher (score ≥ 2) C-PTC (92.9%) F-PTC (52.9%) ATC (33.3%) concerning Interestingly, all C-PTC-BRAFV600E expressed high score for level, strengthening positive correlation between expression. In addition, independent mutational status BRAF, we observed 90% infiltrating showed expression, suggesting may considered complementary biomarker aggressiveness. highly diseases with different subcellular localizations.